GMP Recombinant Human BMP4

• Cat.No.: BMP4-125H
• Product Overview: Disulfide­linked homodimerMouse myeloma cell line, NS0­derivedSer293-­Arg408Accession # Q53XC5Manufactured and tested under current Good Manufacturing Practice (GMP) guidelines.

Specification

• Species: Human
• Source: NS0
• Tag: Non
• Description: BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.
• Predicted N Terminal: Ser293-Pro-Lys-His-His-Ser-Gln-Arg-Ala-Arg
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.The ED50 for this effect is typically 2.5-­15 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 13 kDa (monomer).SDS­PAGE: 22­-25 kDa, reducing conditions; 37­-41 kDa, non­reducing conditions
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS­PAGE with silver staining, under reducing conditions.
• Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles.12 months, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 50-­200 μg/mL in 4 mM HCl.

Gene Information

• Gene Name: BMP4 bone morphogenetic protein 4 [ Homo sapiens ]
• Official Symbol: BMP4
• Synonyms: BMP4; bone morphogenetic protein 4; BMP2B; BMP-4; BMP-2B; bone morphogenetic protein 2B; ZYME; OFC11; BMP2B1; MCOPS6
• Gene ID: 652
• mRNA Refseq: NM_001202
• Protein Refseq: NP_001193
• MIM: 112262
• UniProt ID: Q53XC5
• Chromosome Location: 14q22-q23
• Pathway: Adipogenesis, organism-specific biosystem; Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Glypican 3 network, organism-specific biosystem; Heart Development, organism-specific biosystem; Hedgehog signaling pathway, organism-specific biosystem; Hedgehog signaling pathway, conserved biosystem
• Function: BMP receptor binding; chemoattractant activity; cytokine activity; growth factor activity; protein binding