Fully Active Recombinant Human PLAT Protein

• Cat.No.: PLAT-13H
• Product Overview: Human tPA 85% Single Chain has been recombinantly produced in CHO cells and purified using affinity chromatography.

Specification

• Species: Human
• Source: CHO
• Description: The enzyme is a tetramer containing four gram atoms of copper per molecule (Jolley et al. 1974), and two binding sites for aromatic compounds including phenolic substrates. There is also a distinctly different binding site for oxygen, the copper site (Duckworth and Coleman 1970). The copper is probably in the cuprous state; inactivation of the enzyme is associated with increase in Cu2+. (Kertész et al. 1972). The amino acid composition has been determined. Extensive structural studies have been reported by Jolley et al. (1969); and Duckworth and Coleman (1970). See also Jolley et al. (1972, 1973, and 1974).
• Form: Frozen liquid
• Purity: >95%
• Applications: This product is useful as a positive control for ELISA and western blot and for a variety of in vitro applications including cell differentiation, cell signaling, clotting assays, fibrinolysis, plasminogen activation, protein-protein interactions, receptor binding, thromboelastography, and thrombolysis.
• Storage: At -70 centigrade.
• Concentration: 2.0 mg/mL
• Storage Buffer: 0.4M HEPES, 0.1M NaCl, pH 7.4

Gene Information

• Gene Name: PLAT plasminogen activator, tissue [ Homo sapiens ]
• Official Symbol: PLAT
• Synonyms: PLAT; plasminogen activator, tissue; tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA); TPA; T-PA; DKFZp686I03148
• Gene ID: 5327
• mRNA Refseq: NM_000930
• Protein Refseq: NP_000921
• MIM: 173370
• UniProt ID: P00750