Active Recombinant Human PLAT protein, His-tagged

• Cat.No.: PLAT-1109H
• Product Overview: Recombinant Human PLAT(Met1-Pro562) fused with a C-terminal 6-His tag Single-chain proform was expressed, purified, activated with thermolysin to a 2-chain protease and further purified, was expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: Met1-Pro562
• Predicted N Terminal: Ser36 (Chain A) and Ile311 (Chain B)
• Form: Supplied as a 0.2 μm filtered solution in MES, NaCl and CaCl2.
• Bio-activity: >190 pmol/min/μg,
• Molecular Mass: Predicted Molecular Mass: 31 (Chain A), 29 (Chain B) kDa;SDS-PAGE: 30-40 kDa, reducing conditions
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 centigrade as supplied.3 months, -20 to -70 centigrade under sterile conditions after opening.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: PLAT plasminogen activator, tissue [ Homo sapiens ]
• Official Symbol: PLAT
• Synonyms: PLAT; plasminogen activator, tissue; tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA); TPA; T-PA; DKFZp686I03148
• Gene ID: 5327
• mRNA Refseq: NM_000930
• Protein Refseq: NP_000921
• MIM: 173370
• UniProt ID: P00750
• Chromosome Location: 8p11.21
• Pathway: Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Dissolution of Fibrin Clot, organism-specific biosystem; Endochondral Ossification, organism-specific biosystem; Hemostasis, organism-specific biosystem
• Function: peptidase activity; protein binding; serine-type endopeptidase activity; serine-type endopeptidase activity