ERBB2 protein-coupled Magnetic Beads

• Cat.No.: HER2-006M
• Product Overview: The biotinylated HER2 protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-HER2 antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This HER2 coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.

Specification

• Species: Human
• Source: HEK293
• Protein Length: Thr 23 - Thr 652
• Particle size: 2 μm
• Coupled amount of protein: ≈589 pmol (43 μg) Her2/mg Beads
• Capacity: >180 pmol (27 μg) antibody/mg beads
• Form: PBS, pH7.4, with 10% Trehalose
• Bio-activity: Immobilized 43 μg HER2 protein to 1 mg Beads, can bind the Anti-HER2 Antibody (Herceptin) with an EC50 of 0.7817 μg/mL.
• Applications: This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
• Storage: Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C. Once reconstitution, please aliquote and store under -70°C for no more than 3 months, and avoid repeated freeze-thaw cycles. Immediate use after reconstitution is highly recommended.Please avoid more than 3 freeze-thaw cycles.
• Reconstitution: 2 mL sterile deionized water (1 mg beads/mL)

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.