DNMT3B Chemiluminescent Assay Kit
Cat.No. : | Kit-1716 |
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Tag : | Non |
Size : | 96 reactions |
Description : | The DNMT3B Direct Activity Assay Kit is designed to measure DNMT3B activity for screening and profiling applications. The key to the DNMT3B Direct Activity Assay Kit is a highly specific antibody that recognizes 5-methylcytosine of the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT3B activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT3B/3L for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. |
Applications : | Useful for studying enzyme kinetics and HTS applications. |
Storage : | 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
Kit Components : | DNMT3B/3L: 2 x 20 µg; -80°C400 µM S-adenosylmethionine: 2 x 250 µl; -80°C Anti-5-methylcytosine antibody: 25 µl; -80°C Secondary HRP-labeled antibody 1: 10 µl; -80°C 4x DNMT assay buffer 2: 5 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A (translucent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C Plate pre-coated with DNMT substrate: 1; +4°C |
◆ Recombinant Proteins | ||
DNMT3B-1979H | Recombinant Human DNMT3B Protein (Met1-Ser100), N-His tagged | +Inquiry |
DNMT3B-780H | Recombinant Human DNMT3B Protein, His (Fc)-Avi-tagged | +Inquiry |
Dnmt3b-1646M | Recombinant Mouse Dnmt3b protein, His & T7-tagged | +Inquiry |
DNMT3B-3712H | Recombinant Human DNMT3B protein, GST-tagged | +Inquiry |
Dnmt3b-2622M | Recombinant Mouse Dnmt3b Protein, Myc/DDK-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
DNMT3B-6854HCL | Recombinant Human DNMT3B 293 Cell Lysate | +Inquiry |
Not For Human Consumption!
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