DNMT1 Chemiluminescent Assay Kit
Cat.No. : | Kit-1715 |
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Tag : | Non |
Size : | 96 reactions |
Description : | The DNMT1 Assay Kit is designed to measure DNMT1 activity for screening and profiling applications. The key to the DNM1 Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for DNMT1 detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT1 for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. |
Applications : | Useful for studying enzyme kinetics and HTS applications. |
Storage : | 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
Kit Components : | DNMT1: 2 x 10 µg; -80°C400 µM S-adenosylmethionine: 2 x 250 µl; -80°C Anti-5-methylcytosine antibody: 25 µl; -80°C Secondary HRP-labeled antibody 1: 10 µl; -80°C 4x DNMT assay buffer 1: 5 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A: 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C Strip plate precoated with DNMT1 substrate: 1; +4°C |
◆ Recombinant Proteins | ||
DNMT1-201HFL | Active Recombinant Full Length Human DNMT1 Protein, C-Flag-tagged | +Inquiry |
DNMT1-330H | Recombinant Human DNMT1 Protein, His-Flag-tagged | +Inquiry |
DNMT1-8794Z | Recombinant Zebrafish DNMT1 | +Inquiry |
DNMT1153H | Recombinant Human DNMT1 (621-1616) Protein, His-tagged | +Inquiry |
Dnmt1-139M | Active Recombinant Mouse Dnmt1 | +Inquiry |
Not For Human Consumption!
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