Recombinant Human CYP2C9

• Cat.No.: CYP2C9-537H
• Product Overview: Recombinant Human CYP2C9 allele expressed ininsect cellsis the most common in human populations studied to date and contains Arg, Ile and Gly at positions 144, 359 and 475 respectively.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Description: CYP2C9 is an important cytochrome P450 enzyme with a major role in the oxidation of both xenobiotic and endogenous compounds. CYP2C9 makes up about 18% of the cytochrome P450 protein in liver microsomes. Some 100 therapeutic drugs are metabolized by CYP2C9, including drugs with a narrow therapeutic index such as warfarin and phenytoin and other routinely prescribed drugs such as acenocoumarol, tolbutamide, losartan, glipizide, and some nonsteroidal anti-inflammatory drugs.
• Protein Concentration: 5.5 mg/mL as determined using the BCA assay. Buffer: 100 mM potassium phosphate (pH 7.5), 20% (v/v) glycerol, 0.1 mM EDTA, 1 mM DTT.
• Specific Content: 200 pmol spectral P450 per milligram of protein Cytochrome.
• Reductase Activity: 1.19 μmol of cytochrome c reduced per minute per milligram of protein.
• Hydroxylase Activity: 6.6+0.881 nmol/min/mg of protein. Diclofenac hydroxylation reactions were performed for 10 minutes at 37°C. Reactions were carried out in the presence of 0.1 M Tris HCl (pH 7.5), 100 μM diclofenac, 2 mM NADPH, and at a final protein concentration of 100 μg/mL. The reactions were stopped by addition of 20 μL acetonitrile/glacial acetic acid (96:4).
• Storage: −80℃; Avoid multiple freeze-thaws.
• Safety Precautions: Normal precautions exercised in handling laboratory reagents should be followed. The chemical, physical, and toxicological properties of these products may not, as yet, have been thoroughly investigated. We recommend the use of gloves, lab coats, and eye protection when working with any chemical reagents.

Gene Information

• Gene Name: CYP2C9 cytochrome P450, family 2, subfamily C, polypeptide 9 [ Homo sapiens ]
• Synonyms: CYP2C9; cytochrome P450, family 2, subfamily C, polypeptide 9; CPC9; CYP2C; CYP2C10; MGC88320; P450IIC9; MGC149605; cytochrome P450 2C9; CYPIIC9; cytochrome P-450MP; cytochrome p4502C9; cytochrome P450 MP-4; cytochrome P450 MP-8; cytochrome P450 PB-1; cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 9; cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 10; EC 1.14.13.48; EC 1.14.13.49; EC 1.14.13.80
• Gene ID: 1559
• mRNA Refseq: NM_000771
• Protein Refseq: NP_000762
• MIM: 601130
• UniProt ID: P11712
• Chromosome Location: 10q24
• Pathway: Arachidonic acid metabolism; Drug metabolism - cytochrome P450; Linoleic acid metabolism; Metabolic pathways; Metabolism of xenobiotics by cytochrome P450; Retinol metabolism
• Function: (S)-limonene 6-monooxygenase activity; (S)-limonene 7-monooxygenase activity; 4-hydroxyacetophenone monooxygenase activity; caffeine oxidase activity; electron carrier activity; monooxygenase activity; oxidoreductase activity; steroid hydroxylase activity