Active Recombinant Mouse Cd274, Fc-tagged, Biotinylated

• Cat.No.: Cd274-721M
• Product Overview: The recombinant mouse PD-L1/CD274 ECD is expressed as a 458 amino acid protein consisting of Phe19 - Thr238 region of mouse PD-L1/CD274 (UniProt accession #Q9EP73) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

Specification

• Species: Mouse
• Source: Human Cells
• Tag: Fc
• Protein Length: 19-238 a.a.
• Form: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule).
• Bio-activity: Binds to its receptor PD-1 and anti-PD-L1 monoclonal antibody. Blocks PD-L1"s binding to its receptor and mediated signaling activity. Shows cross-species binding to human PD-1 and human CD80 in a functional ELISA
• Molecular Mass: Calculated molecular mass 51.5 kDa; estimated by SDS-PAGE under reducing condition 60-65 kDa suggesting that the protein is highly glycosylated
• Endotoxin: <0.1 eu="" per="" 1="" μg="" of="" purified="" recombinant="" protein="" determined="" by="" the="" lal="">
• Purity: >95% judged by SDS-PAGE under reducing condition
• Storage: The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Cd274 CD274 antigen [ Mus musculus ]
• Official Symbol: Cd274
• Synonyms: CD274; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Gene ID: 60533
• mRNA Refseq: NM_021893
• Protein Refseq: NP_068693
• Pathway: Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; PD-1 signaling, organism-specific biosystem
• Function: protein binding; receptor activity