Active Recombinant Human USP1&WDR48 Protein, His-tagged

• Cat.No.: USP1&WDR48-1067H
• Product Overview: Recombinant Human USP1&WDR48 (Accession # O94782 (USP1), Q8TAF3 (UAF1)), fused with a C-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: His
• Form: X mg/ml total protein including USP1 (X µM) and UAF1(X µM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP
• Bio-activity: Recombinant Human USP1/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1/UAF1 complex concentration of 0.01-0.5 µM.
• Molecular Mass: Recombinant Human USP1&WDR48, His-tagged has a calculated MW of 89 kDa (USP1), 77 kDa (UAF1).
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: USP1 ubiquitin specific peptidase 1 [ Homo sapiens ]
• Official Symbol: USP1
• Synonyms: USP1; ubiquitin specific peptidase 1; ubiquitin specific protease 1; ubiquitin carboxyl-terminal hydrolase 1; hUBP; ubiquitin thioesterase 1; deubiquitinating enzyme 1; ubiquitin thiolesterase 1; ubiquitin carboxyl terminal hydrolase 1; ubiquitin specific processing protease 1; ubiquitin-specific-processing protease 1; UBP
• Gene ID: 7398
• mRNA Refseq: NM_001017415
• Protein Refseq: NP_001017415
• MIM: 603478
• UniProt ID: O94782
• Chromosome Location: 1p31.3