Active Recombinant Human USP1&WDR48 Protein, His-tagged

• Cat.No.: USP1&WDR48-1067H
• Product Overview: Recombinant Human USP1&WDR48 (Accession # O94782 (USP1), Q8TAF3 (UAF1)), fused with a C-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Human
• Tag: His
• Form: X mg/ml total protein including USP1 (X µM) and UAF1(X µM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP
• Bio-activity: Recombinant Human USP1/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1/UAF1 complex concentration of 0.01-0.5 µM.
• Molecular Mass: Recombinant Human USP1&WDR48, His-tagged has a calculated MW of 89 kDa (USP1), 77 kDa (UAF1).
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: USP1 ubiquitin specific peptidase 1 [ Homo sapiens ]
• Official Symbol: USP1
• Synonyms: USP1; ubiquitin specific peptidase 1; ubiquitin specific protease 1; ubiquitin carboxyl-terminal hydrolase 1; hUBP; ubiquitin thioesterase 1; deubiquitinating enzyme 1; ubiquitin thiolesterase 1; ubiquitin carboxyl terminal hydrolase 1; ubiquitin specific processing protease 1; ubiquitin-specific-processing protease 1; UBP
• Gene ID: 7398
• mRNA Refseq: NM_001017415
• Protein Refseq: NP_001017415
• MIM: 603478
• UniProt ID: O94782
• Chromosome Location: 1p31.3

Citation

Efficient Zygotic Genome Editing via RAD51-Enhanced Interhomolog Repair

Journal: bioRxiv    Data: 2018/8/6

Authors: Wilde Jonathan J., Aida Tomomi, Feng Guoping

Article Snippet:PrePrint: For experiments using wildtype recombinant proteins, the following proteins were used: RAD51 (Creative BioMart RAD51-134H), USP1/WDR48 (Creative BioMart USP1&WDR48-1067H), BCCIP (Origene TP303061), XRCC1 (TP304952), XRCC4 (Origene TP312684), DMC1 (Origene TP318311).. For experiments using mutant RAD51, custom RAD51 preparations were produced by Creative BioMart according to their standard protocol for producing the wildtype RAD51 used in all other experiments.For experiments using mutant RAD51, custom RAD51 preparations were produced by Creative BioMart according to their standard protocol for producing the wildtype RAD51 used in all other experiments.

Publication :

Efficient Zygotic Genome Editing via RAD51-Enhanced Interhomolog Repair (2018)