Recombinant Mouse Glutathione S-transferase Mu 1, His-tagged

• Cat.No.: Gstm1-124M
• Product Overview: Recombinant GSTM1 protein was expressed in E.coli and purified by using conventional chromatography techniques, 25.9 kDa (218 aa) (Real molecular weight on SDS-PAGE will be shift up).

Specification

• Cat. No.: Gstm1-124M
• Description: GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress.
• Sequences of amino acids: MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK
• Formulation: Liquid. In PBS (pH7.4) containing 5 mM glutathione
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method)
• Biological activity: Specific activity is 3 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.
• Activity Assay: 1. Prepare a 1.mll reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25°C and monitor the A340nm until the value is constant using a spectrophotometer. 3. Add 50ug of recombinant mouse GSTM1 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Record the increase in A340nm for 5 minutes.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: Gstm1 glutathione S-transferase, mu 1 [ Mus musculus ]
• Synonyms: EC 2.5.1.18; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; H-B; GST1; MGC26563; MU; OTTHUMP00000013346; OTTHUMP00000013347; GST class-mu 1; HB subunit 4; S-(hydroxyalkyl)glutathione lyase; glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; glutathione S-transferase mu 1; Gstb1; Gstb-1; Gstm1; glutathione S-transferase mu 1; GTM1; MU-1; OTTMUSP00000007628; glutathione-S-transferase, mu 1
• Gene ID: 14862
• mRNA Refseq: NM_010358
• Protein Refseq: NP_034488
• UniProt ID: P10649
• Chromosome Location: 3 F2.3
• Pathway: Glutathione metabolism; Metabolism of xenobiotics by cytochrome P450
• Function: glutathione transferase activity ; transferase activity