Recombinant Human AR, His-tagged

• Cat.No.: AR-17H
• Product Overview: Recombinant Human AR(Glu2-Gln556), fused with His x6 at N-terminus, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-556 a.a.
• Description: The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.
• Form: Liquid, clear solution. Supplied as a 0.2 µm filtered solution of 0.1 M NaHCO3, 0.5 M NaCl, pH 7.5.
• Molecular Mass: 62 kDa
• Endotoxin: Not Determined
• Purity: >95%
• Applications: ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array, Function Assay, etc. Before use, heat the WBC control at 95 °C for 5 minutes. Spin down the sample and load 5 µL per lane onto SDS-PAGE gel.

Gene Information

• Gene Name: AR androgen receptor [ Homo sapiens (human) ]
• Official Symbol: AR
• Synonyms: AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA; androgen receptor; dihydrotestosterone receptor; androgen nuclear receptor variant 2; nuclear receptor subfamily 3 group C member 4
• Gene ID: 367
• mRNA Refseq: NM_000044
• Protein Refseq: NP_000035
• MIM: 313700
• UniProt ID: P10275
• Chromosome Location: Xq12
• Pathway: Alpha6-Beta4 Integrin Signaling Pathway; Androgen receptor signaling pathway; FOXA1 transcription factor network
• Function: DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription