Recombinant Human CHK1 Checkpoint Homolog (S. pombe), GST-tagged

• Cat.No.: CHEK1-4838H
• Product Overview: Recombinant full-length human CHEK1 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: CHEK1 is a 56 kd serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle. CHEK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 82 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 Mm EDTA, 0.1 mM PMSF, 25 % glycerol.
• Specific Activity: 214 nmol/min/mg
• Purity: > 95 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Activation of ATR in response to replication stress; Cell cycle; p53 signaling pathway; Cell Cycle Checkpoints; IL-3 Signaling Pathway; HTLV-I infection; Ubiquitin Mediated Degradation of Phosphorylated Cdc25A

Gene Information

• Gene Name: CHEK1 CHK1 checkpoint homolog (S. pombe) [ Homo sapiens ]
• Official Symbol: CHEK1
• Synonyms: CHEK1; CHK1 checkpoint homolog (S. pombe); CHK1; CHK1 (checkpoint, S. pombe) homolog; Serine/threonine-protein kinase Chk1; EC 2.7.11.1; serine/threonine-protein kinase Chk1; OTTHUMP00000231300; OTTHUMP00000231301; OTTHUMP00000231306; OTTHUMP00000231307; OTTHUMP00000231309; Checkpoint, S. pombe, homolog of, 1
• Gene ID: 1111
• mRNA Refseq: NM_001114121
• Protein Refseq: NP_001107593
• MIM: 603078
• UniProt ID: O14757
• Chromosome Location: 11q24.2
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity