Recombinant Human ACP1 protein(Met1-His158), GST-tagged

• Cat.No.: ACP1-1146H
• Product Overview: Recombinant Human ACP1 (AAI06012.1) (Met 1-His 158) was expressed in E. coli, fused with the GST tag at the N-terminus.

Specification

• Product Overview: Recombinant Human ACP1 (AAI06012.1) (Met 1-His 158) was expressed in E. coli, fused with the GST tag at the N-terminus.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: 1-158 a.a.
• Form: Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >65, 000 pmol/min/μg.
• Molecular Mass: The recombinant human ACP1/GST chimera consists of 384 amino acids and predicts a molecular mass of 44.3 kDa. The apparent molecular mass of rh ACP1 is approximately 40 kDa in SDS-PAGE under reducing conditions.
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ACP1 acid phosphatase 1, soluble [ Homo sapiens ]
• Official Symbol: ACP1
• Synonyms: ACP1; acid phosphatase 1, soluble; low molecular weight phosphotyrosine protein phosphatase; LMW-PTP; LMW-PTPase; adipocyte acid phosphatase; red cell acid phosphatase 1; protein tyrosine phosphatase; acid phosphatase of erythrocyte; cytoplasmic phosphotyrosyl protein phosphatase; low molecular weight cytosolic acid phosphatase; HAAP; MGC3499; MGC111030
• Gene ID: 52
• mRNA Refseq: NM_001040649
• Protein Refseq: NP_001035739
• MIM: 171500
• UniProt ID: P24666

Case Study

Case 1: Faria AVS, et al. Mol Cell Biochem. 2020

Over the past decade, studies have shown that low molecular weight protein tyrosine phosphatase (LMWPTP) plays a big role in making cancer more aggressive and resistant, especially in chronic myeloid leukemia. Here, LMWPTP keeps Src and Bcr-Abl kinases active and shifts glucose metabolism towards lactate production, supporting the pentose pathway, which is key for antioxidant defense. This study explores the link between LMWPTP and autophagy. In resistant chronic myeloid leukemia cells, the glycolytic metabolism, alongside antioxidant enzymes like SOD and catalase, backed by LMWPTP, boosts the antioxidant response. However, when exposed to hydrogen peroxide, the level of LMWPTP and SOD decreases, triggering autophagy.

Fig1. LMWPTP expression at chronic myeloid leukemia cells and autophagy-related proteins.

Fig2. LMWPTP expression coordinates autophagy.

Case 2: Faria AVS, et al. Biochim Biophys Acta Mol Basis Dis. 2022

In recent years, some proteins in the tyrosine phosphatase family have been recognized as players in cancer growth. Specifically, Low Molecular Weight Protein Tyrosine Phosphatase (LMWPTP) has been linked to liver spread in colorectal cancer and worse outcomes in prostate cancer. Interestingly, how cancer cells interact with platelets seems to be key in cancer getting worse and spreading, with platelets believed to boost several harmful traits of tumors. The research aimed to see how LMWPTP levels in cancer cells impact their interaction with platelets. The gene for this phosphatase is overactive in upper gastrointestinal (GI) and colorectal cancer cells and gets even more active when platelets are around. This interaction seems to encourage the growth of GI tumor cells. Plus, by reducing LMWPTP levels in cancer cells, these cells interacted less with platelets and slowed down platelet-driven growth.

Fig1. Kinase activation (Src and p38), and LMWPTP expression in normal and cancer gastric cell line.

Fig2. Light microscopy images of HT29 cells showing reduced platelet aggregates upon KO of LMWPTP.

Application

Human ACP1, also called low molecular weight tyrosine phosphatase (LMWPTP), is a crucial enzyme in the cell cytoplasm. It's heavily involved in controlling cell signals, particularly those related to cell growth, specialization, and metabolism. ACP1 is key in managing how proteins get phosphorylated, which ties it to immune responses, cancer development, and autoimmune conditions. Research indicates that variations in the ACP1 gene might influence how prone someone is to certain illnesses, making it a promising marker for personalized medicine. Recombinant Human ACP1 protein, known for its role as a low molecular weight tyrosine phosphatase, is widely used in various fields due to its regulatory functions in cell signaling, growth, and metabolism. In scientific research, it's employed to study cellular processes and immune response mechanisms. In the medical field, ACP1 is explored for its potential as a biomarker for certain diseases, aiding in personalized medicine approaches. Additionally, its involvement in protein tyrosine phosphorylation makes it valuable for cancer research and understanding autoimmune conditions. This versatility in applications highlights ACP1's importance across research and industrial sectors.

Fig1. LMWPTP acts as a hub in cancer hallmarks. (Alessandra V S Faria, 2021)