Recombinant Mouse EGFR protein(Met1-Ser647), hFc-tagged

• Cat.No.: EGFR-102M
• Product Overview: Recombinant Mouse EGFR (Q01279) (Met 1-Ser 647) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Protein length: 1-647 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind human EGF in a functional ELISA.
• Molecular Mass: The secreted recombinant mouse EGFR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 864 amino acids and has a calculated molecular mass of 96 kDa. As a result of glycosylation, the apparent molecular mass of rmEGFR/Fc monomer is approximately 130 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Egfr epidermal growth factor receptor [ Mus musculus ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; waved 2; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; Wa5; wa2; Erbb; Errp; wa-2; AI552599; 9030024J15Rik
• Gene ID: 13649
• mRNA Refseq: NM_007912
• Protein Refseq: NP_031938