Cathepsin K Inhibitor Screening Kit (Fluorometric)

• Cat.No.: Kit-2352
• Product Overview: Cathepsin K (CTSK, EC 3.4.22.38), a lysosomal cysteine protease, is involved in osteoclastic bone remodeling and resorption. In addition, it also degrades collagen, gelatin and elastin. Cathepsin K Inhibitor Screening Kit utilizes the ability of active Cathepsin K to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin K-specific inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin K.

Specification

• Tag: Non
• Applications: Screen/study/characterize potential inhibitors of Cathepsin K.
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: CTSK Reaction Buffer; CTSK Reagent; Cathepsin K (Human); CTSK Substrate, Ac-LR-AFC (10 mM); CTSK Inhibitor (FF-FMK, 1 mM)
• Detection method: Fluorescence (Ex/Em = 400/505 nm)
• Features & Benefits: Simple & convenient;
  High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin K;
  Cathepsin K inhibitor, FF-FMK is included in the kit