Cathepsin E Fluorometric Activity Assay Kit

• Cat.No.: Kit-2145
• Product Overview: Cathepsin E Activity Assay Kit utilizes the ability of an active Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. This assay kit is simple, rapid and can detect Cathepsin E activity as low as 1 mU in biological samples.

Specification

• Tag: Non
• Size: 100 assays
• Description: Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers.
• Applications: Detection of Cathepsin E activity in tissue and cell lysatesDetermine activity of purified Cathepsin E
• Storage: Store kit at -20°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read entire protocol before performing the assay.CTSE Assay Buffer and Lysis Buffer: Bring to room temperature before use. Store at -20°C.Human Cathepsin E: Reconstitute with 20 µl of de-ionized water. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Use within two months.
• Kit Components: CTSE Assay Buffer: 25 mlCTSE Lysis Buffer: 25 mlCTSE Substrate: 0.2 mlHuman Cathepsin E (Positive Control): 1 vialMCA Standard (1 mM): 15 μl
• Detection method: Fluorescence (Ex/Em = 320/420 nm)
• Compatible Sample Types: • Cell and tissue lysates• Purified Cathepsin E
• Features & Benefits: • Rapid & convenient• Detects Cathepsin E activity as low as 1 mU in biological samples