Casein, TAMRA-conjugated

• Cat.No.: DYE-137

Specification

• Tag: Non
• Description: In the intact substrate, casein is heavily labeled with TAMRA, resulting in almost total quenching of the conjugate"s fluorescence. Compared to the fluoresceinated casein, TAMRA-labeled casein demonstrates pH-insensitive fluorescence. It is readily used for continuous fluorometric measurement of protease activity. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly red fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. The lightly TAMRA-labeled casein may be useful for a continuous assay if monitored by fluorescence polarization.
• Ex(nm): 545
• Em(nm): 576
• Solvent: Water
• Storage: Store at -20°C in the dark. Dessicated.