Casein, FITC-conjugated

• Cat.No.: DYE-136

Specification

• Tag: Non
• Description: Fluoresceinated casein is widely used for fluorometric measurement of protease activity. In the intact substrate, casein is heavily labeled with a fluorescein derivative, resulting in almost total quenching of the conjugate""s fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. The lightly labeled fluorescein casein may be useful for a continuous assay if monitored by fluorescence polarization.
• Ex(nm): 494
• Em(nm): 521
• Solvent: Water
• Storage: Store at -20°C in the dark. Dessicated.