BRD3 (BD1+BD2) TR-FRET Assay Kit
Cat.No. : | Kit-1694 |
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Tag : | Non |
Size : | 384 reactions |
Description : | The BRD3 (BD1+BD2) TR-FRET Assay Kit is designed to measure the inhibition of BRD3 (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD3, ligand, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader. |
Applications : | Great for screening small molecular inhibitors for drug discovery and HTS applications. |
Storage : | At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
Kit Components : | BRD3 (BD1+BD2): 10 µg; -80°CBET Bromodomain Ligand: 50 µl; -80°C Non-acetylated Ligand 1: 15 µl; -20°C Tb-donor: 2x10 µl; -20°C Dye-labeled acceptor: 2x10 µl; -20°C 3x BRD TR-FRET Assay Buffer 1: 4 ml; -20°C White Nonbinding low volume microtiter plate: 1; Room temp. |
◆ Recombinant Proteins | ||
BRD3-2839H | Recombinant Human BRD3 protein, His&FLAG-tagged | +Inquiry |
BRD3-2838H | Recombinant Human BRD3 protein, His&FLAG-tagged | +Inquiry |
BRD3-2837H | Recombinant Human BRD3(BD1+BD2) protein, His-tagged | +Inquiry |
BRD3-563R | Recombinant Rhesus monkey BRD3 Protein, His-tagged | +Inquiry |
BRD3-688H | Recombinant Human bromodomain containing 3, His-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
BRD3-178HCL | Recombinant Human BRD3 cell lysate | +Inquiry |
Not For Human Consumption!
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