Bovine Intestinal Alkaline Phosphatase

• Cat.No.: BIAP-74B
• Product Overview: The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity. Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.

Specification

• Species: Bovine
• Source: Calf Intestine
• Tag: Non
• Physical Appearance: Sterile Filtered clear colorless solution (11.1 mg/ml).
• Purity: 95% pure by Gel Filtration.
• Formulation: 50% glycerol, 5 mM MgCl2, 0.1 mM ZnCl2 and 5 mM TRIS, pH 7.0.
• Biological Activity: >1500 U/mg (pH 9.6, 37°C and 0.025 M glycin, 10% glycerol as buffer).
• Stability: AP although stable at 14°C for 1 weeks, should be stored at 4°C. Please Do-Not freeze.

Gene Information

• Synonyms: EC 3.1.3.1; IAP; AP; Alk_phosphatase; glycerophosphatase; Kasahara isozyme; alkaline phosphatase, intestinal; alkaline phosphomonoesterase; intestinal alkaline phosphatase; alkaline phosphatase; alkaline phosphomonoesterase; phosphomonoesterase; glycerophosphatase; alkaline phosphohydrolase; alkaline phenyl phosphatase; orthophosphoric-monoester phosphohydrolase (alkaline optimum); phosphate-monoester phosphohydrolase (alkaline optimum).