bmMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate

• Cat.No.: bmMSCs-172H

Specification

• Species: Human
• Tag: Non
• Tissue Type: Bone Marrow
• Preparation method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Size: 50 ug
• Recommended Usage: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Storage Buffer: In modified RIPA Lysis Buffer.
• Storage Instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Applications: Western Blot;Immunoprecipitation