Active Recombinant SV40gp6, His-tagged

• Cat.No.: SV40gp6-8506S
• Product Overview: Recombinant SV40gp6,fused with His tag, was expressed in Sf21 Cells.

Specification

• Species: Simian virus
• Source: Sf21 Cells
• Tag: His
• Description: The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth . A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain . The p53-interacting domain is also essential for binding p300/CBP . Phosphorylation of TAg by several kinases may regulate its function both positively and negatively.
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• Bio-activity: 1 unit equals 1 ng of purified protein. 50-500 units are sufficient for an in vitro unwinding assay and 100 units are sufficient for a protein-protein interaction assay.
• AA Sequence: MDKVLNREES LQLMDLLGLE RSAWGNIPLM RKAYLKKCKE FHPDKGGDEE KMKKMNTLYK KMEDGVKYAH QPDFGGFWDA TEIPTYGTDE WEQWWNAFNE ENLFCSEEMP SSDDEATADS QHSTPPKKKR KVEDPKDFPS ELLSFLSHAV FSNRTLACFA IYTTKEKAAL LYKKIMEKYS VTFISRHNSY NHNILFFLTP HRHRVSAINN YAQKLCTFSF LICKGVNKEY LMYSALTRDP FSVIEESLPG GLKEHDFNPE EAEETKQVSW KLVTEYAMET KCDDVLLLLG MYLEFQYSFE MCLKCIKKEQ PSHYKYHEKH YANAAIFADS KNQKTICQQA VDTVLAKKRV DSLQLTREQM LTNRFNDLLD RMDIMFGSTG SADIEEWMAG VAWLHCLLPK MDSVVYDFLK CMVYNIPKKR YWLFKGPIDS GKTTLAAALL ELCGGKALNV NLPLDRLNFE LGVAIDQFLV VFEDVKGTGG ESRDLPSGQG INNLDNLRDY LDGSVKVNLE KKHLNKRTQI FPPGIVTMNE YSVPKTLQAR FVKQIDFRPK DYLKHCLERS EFLLEKRIIQ SGIALLLMLI WYRPVAEFAQ SIQSRIVEWK ERLDKEFSLS VYQKMKFNVA MGIGVLDWLR NSDDDDEDSQ ENADKNEDGG EKNMEDSGHE TGIDSQSQGS FQAPQSSQSV HDHNQPYHIC RGFTCFKKPP TPPPEPET
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 400 μg/ml
• Shipping: Dry Ice

Gene Information

• Gene Name: SV40gp6 large T antigen [ Simian virus 40 ]
• Official Symbol: SV40gp6
• Synonyms: SV40gp6; large T antigen
• Gene ID: 1489531
• Protein Refseq: YP_003708382