| Species : |
Serratia marcescens |
| Source : |
Yeast |
| Description : |
Binuclease is a genetically engineered endonuclease from Serratia marcescens gene that can digest all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. Binuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester. |
| Bio-activity : |
≥ 20 kU/mg lyophilized powder; ≥ 250 U/μL buffered aqueous glycerol solution |
| Purity : |
Grade I: ≥90%; (SDS-PAGE); Grade II (superior purity): ≥95%; (SDS-PAGE); Protease and Endotoxin (Tachypleus Amebocyte Lysate) : None Detected. |
| Unit Definition : |
One unit of Binuclease will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 minutes at pH 8.0 at 37 centigrade. |
| Applications : |
Our Binuclease Endonuclease cleaves all forms of DNA and RNA, which makes it an ideal tool to eliminate nucleic acids (DNA and RNA) contamination in vaccine, antibody, cell therapy and other biological products for therapeutic use to meet FDA guidelines that nucleic acid contamination should be limited to 100 pg per dose in the end product. Binuclease reduces nucleic acid load and viscosity in cell lysates during purification to facilitate the filtration/ultrafiltration of solutions, reduce processing time, improve the separation of pellets and supernatants in centrifugations and increase the efficiency of chromatographic purification, resulting significant improvements on both yield and purity of cell-derived particles such as viruses, AAV vectors or inclusion bodies. Bioanalytical applications: Sample preparation for ELISA, chromatography or two- dimensional electrophoresis (protein mapping), and footprint analysis to get improved resolution and increased recovery of samples. |
| Stability : |
Lyophilized powder is stable for 3 years when stored below 4 centigrade. Buffered aqueous glycerol solution is stable for 1 year when stored at -20 centigrade. |
| Storage : |
At -20 centigrade |
| Storage Buffer : |
The product is available as lyophilized powder and also as buffered aqueous glycerol solution. 20mM Tris-HCl (pH 8.0), 2mM MgCl2, 20mM NaCl, 50% glycerol |
| Dilutions : |
20mM Tris-Cl (pH 8.0), 2mM MgCl2, 20mM NaCl |
| Shipping : |
Shipped at Room Temperature. |
