Active Recombinant SARS-CoV-2 S Protein, mFc-tagged

• Cat.No.: S-01S
• Product Overview: Recombinant 2019-nCoV S Protein RBD is produced by our Mammalian expression system and the target gene encoding Asn331-Val524 is expressed with a mFc tag at the C-terminus.

Specification

• Species: SARS-CoV-2
• Source: Human Cells
• Tag: mFc
• Protein Length: 331-524 a.a.
• Description: The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
• Form: Supplied as a 0.2 μM filtered solution of PBS, pH 7.4.
• Bio-activity: Immobilized Human ACE-2-His at 10μg/ml (100 μl/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein RBD-mFc. The ED50 of SARS-CoV-2 (2019-nCoV) Spike Protein RBD-mFc is 62 ng/ml.
• Molecular Mass: Predicted Molecular Weight: 47.9kDa
  Apparent Molecular Weight: 50-60kDa, reducing conditions.
• AA Sequence: Asn331-Val524
• Endotoxin: NA
• Purity: > 95%
• Applications: Immunogen, calibrator or standard.
• Usage: Research Use Only
• Storage: Reconstituted protein solution should be stored at ≤ -20 centigrade.
• Quality Statement: Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped on dry ice pack.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: S
• Synonyms: S protein RBD; 2019-nCoV S protein RBD; spike glycoprotein
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2