Active Recombinant Saccharomyces cerevisiae UBC13, His-tagged

• Cat.No.: UBC13-116Y
• Product Overview: Recombinant Saccharomyces cerevisiae UBC13, fused with His tag, was purified from Saccharomyces cerevisiae.

Specification

• Source: Saccharomyces cerevisiae
• Species: Saccharomyces cerevisiae
• Tag: His
• Form: Liquid, 50mM HEPES, ph7.5, 150 mM NaCl, 10% glycerol
• Bio-activity: Typical enzyme concentration of 100nM-5mM is used for in vitro conjugation, depending on conditions
• Molecular Mass: 17kDa
• Purity: ≥ 95% by SDS-PAGE
• Applications: Identify cognate E2/E3 pairsCharacterize upregulation/downregulation of E2 enzymesBuild poly-ubiquitin chainsCharacterize ubiquitin chain specificity of E2 enzymesAnalyze expression of E2 enzymes
• Storage: Store at -80℃. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: UBC13 E2 ubiquitin-conjugating protein UBC13 [ Saccharomyces cerevisiae S288c ]
• Official Symbol: UBC13
• Synonyms: E2 ubiquitin-conjugating protein UBC13
• Gene ID: 851666
• mRNA Refseq: NM_001180400
• Protein Refseq: NP_010377
• UniProt ID: P52490
• Chromosome Location: chromosome: IV
• Pathway: Ubiquitin mediated proteolysis, organism-specific biosystem; Ubiquitin mediated proteolysis, conserved biosystem
• Function: ATP binding; ligase activity; nucleotide binding