Active Recombinant Rhesus NKG2D Protein, His-tagged

• Cat.No.: NKG2D-1201R
• Product Overview: Recombinant rhesus NKG2D (NP_001028061.1) (Phe78-Val216) was expressed with a polyhistide tag at the N-terminus.

Specification

• Species: Rhesus macaque
• Source: Insect Cells
• Tag: His
• ProteinLength: Phe78-Val216
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human ULBP1-Fch, The EC50 of human ULBP1-Fch is 0.12-0.28 μg/ml.
  2. Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch, The EC50 of human MICB-Fch is 33.8-79 ng/ml.
• Molecular Mass: The recombinant rhesus NKG2D consists of 155 amino acids and has a calculated molecular mass of 18.3 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.