Active Recombinant Rhesus IFNA2 protein, His-tagged

• Cat.No.: IFNA2-231R
• Product Overview: Recombinant Rhesus IFNA2 protein(NP_001129266.1) (Met1-Glu188) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: His
• Protein Length: Met1-Glu188
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured in antiviral assay using WISH human amnion cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 0.4-4 pg/mL.
  2. Measured by its binding ability in a functional ELISA. Immobilized Rhesus IFNA2-His at 10 μg/ml (100 μl/well) can bind human IFNAR2-Fc with a linear range of 0.31-2.5 μg/ml.
• Molecular Mass: The recombinant rhesus IFNA2 comprises 176 amino acids and has a calculated molecular mass of 21 KDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.