Active Recombinant Rhesus ICOS protein, hFc-tagged

• Cat.No.: ICOS-1015R
• Product Overview: Recombinant Rhesus ICOS protein(NP_001253918.1)(Met1-Lys140), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: Fc
• Protein Length: 1-140 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized Recombinant Rhesus ICOS / AILIM / CD278 Protein (Fc Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Cynomolgus ICOS Ligand / B7-H2 / ICOSLG Protein (ECD,His Tag),The EC50 is 3-12 ng/mL.
• Molecular Mass: The recombinant rhesus ICOS consists of 378 amino acids and predicts a molecular mass of 42.7 kDa.
• Proteinlength: Met1-Lys140
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.