Active Recombinant Rat Tnfrsf1a protein(Met1-Ala211), hFc-tagged

• Cat.No.: Tnfrsf1a-7449R
• Product Overview: Recombinant Rat TNFRSF1A (P22934) (Met1-Ala211) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Ala211
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 ng/mL in the presence of 0.05 ng/mL of RatTNFα. 2. Immobilized mouse TNFa (80-235) at 10 μg/ml (100 μl/well) can bind Rat TNFRSF1A-Fc, The EC50 of Rat TNFRSF1A-Fc is 0.08-0.20 μg/ml.
• Molecular Mass: The recombinant Rat TNFRSF1A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 431 amino acids and has a predicted molecular mass of 47.9 kDa. The apparent molecular mass of the protein is approximately 59 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tnfrsf1a tumor necrosis factor receptor superfamily, member 1a [ Rattus norvegicus ]
• Official Symbol: Tnfrsf1a
• Synonyms: TNFRSF1A; tumor necrosis factor receptor superfamily, member 1a; tumor necrosis factor receptor superfamily member 1A; p55; p60; TNF-R1; TNF-RI; TNFR-I; tumor necrosis factor receptor 1; tumor necrosis factor receptor type I; Tnfr1; MGC105478
• Gene ID: 25625
• mRNA Refseq: NM_013091
• Protein Refseq: NP_037223