Active Recombinant Rat Serpine1 protein, hFc-tagged

• Cat.No.: Serpine1-564R
• Product Overview: Recombinant Rat Serpine1 protein(NP_036752.1)(Met1-Pro402), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Pro402
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50 value is < 10 nM.
• Molecular Mass: The recombinant rat SERPINE1 comprises 620 amino acids and has a predicted molecular mass of 69.6 kDa. The apparent molecular mass of the protein is approximately 70 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Serpine1 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 [ Rattus norvegicus ]
• Official Symbol: Serpine1
• Synonyms: SERPINE1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; plasminogen activator inhibitor 1; PAI; PAI-1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, member 1; serine (or cysteine) peptidase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; Pai1; PAI1A; Planh; Pai1aa; RATPAI1A
• Gene ID: 24617
• mRNA Refseq: NM_012620
• Protein Refseq: NP_036752