Active Recombinant Rat Rtn4 protein, IgG1-tagged

• Cat.No.: Rtn4-924R
• Product Overview: Recombinant Rat Rtn4(Thr544 - Phe725) fused with Human IgG1(Pro100 - Lys330) at C-terminal was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Rat
• Tag: Fc
• Predicted N Terminal: Thr544
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 300 ng on a nitrocellulose-coated microplate.
• Molecular Mass: Predicted Molecular Mass: 46.1 (monomer) kDa;SDS-PAGE: 75-85 kDa, reducing conditions.
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Protein length: 544-725 a.a.

Gene Information

• Gene Name: Rtn4 reticulon 4 [ Rattus norvegicus ]
• Official Symbol: Rtn4
• Synonyms: RTN4; reticulon 4; reticulon-4; foocen; NI-220/250; nogo protein; GLUT4 vesicle 20kDa protein; neurite outgrowth inhibitor; glut4 vesicle 20 kDa protein; r; Nogo; Vp20; rat N; NI-250; Nogo-A; rat NogoA; MGC116054
• Gene ID: 83765
• mRNA Refseq: NM_031831
• Protein Refseq: NP_114019
• Chromosome Location: 14q22
• Pathway: Axonal growth inhibition (RHOA activation), organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signalling by NGF, organism-specific biosystem; p75 NTR receptor-mediated signalling, organism-specific biosystem; p75NTR regulates axonogenesis, organism-specific biosystem
• Function: protein binding; protein complex binding