Active Recombinant Rat Pde7a protein, GST-tagged

• Cat.No.: Pde7a-1543R
• Product Overview: Recombinant Rat Pde7a(147–482 aa) fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Source: Insect cells
• Species: Rat
• Tag: GST
• Form: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT.
• Bio-activity: ≥354 pmol/min/µg.
• Molecular Mass: 65 kDa
• Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37 centigrade.
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: >6 months at -80 centigrade. Avoid freeze/thaw cycles.
• Concentration: 0.34 mg/ml
• Protein length: 147-482 a.a.

Gene Information

• Gene Name: Pde7a phosphodiesterase 7A [ Rattus norvegicus ]
• Official Symbol: Pde7a
• Synonyms: PDE7A; phosphodiesterase 7A; high affinity cAMP-specific 3,5-cyclic phosphodiesterase 7A; rolipram-insensitive phosphodiesterase type 7; PDE7-1
• Gene ID: 81744
• mRNA Refseq: NM_031080
• Protein Refseq: NP_112342
• Chromosome Location: 2q24
• Pathway: G Protein Signaling Pathways, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Morphine addiction, organism-specific biosystem; Morphine addiction, conserved biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem
• Function: 3,5-cyclic-AMP phosphodiesterase activity; 3,5-cyclic-AMP phosphodiesterase activity; 3,5-cyclic-AMP phosphodiesterase activity; cyclic-nucleotide phosphodiesterase activity; hydrolase activity; metal ion binding; phosphoric diester hydrolase activity