Active Recombinant Rat NTRK2 Protein, His-tagged
Cat.No. : | NTRK2-1095R |
Product Overview : | Recombinant rat NTRK2 (Q63604-Isoform T1) (Met1-His429) was expressed with a polyhistidine tag at the C-terminus. |
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Species : | Rat |
Source : | HEK293 |
Tag : | His |
ProteinLength : | 1-429 a.a. |
Predicted N Terminal : | Cys 32 |
Form : | Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol. |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized rat NTRK2-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse BDNF, The EC50 of biotinylated mouse BDNF is 12.2-28.6 ng/ml. |
Molecular Mass : | The recombinant rat NTRK2 comprises 409 amino acids and predicts a molecular mass of 47.1 kDa. The apparent molecular mass of the recombinant protein is approximately 67 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | >95 % as determined by SDS-PAGE. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name | Ntrk2 neurotrophic tyrosine kinase, receptor, type 2 [ Rattus norvegicus ] |
Official Symbol | NTRK2 |
Synonyms | NTRK2; neurotrophic tyrosine kinase, receptor, type 2; BDNF/NT-3 growth factors receptor; trk-B; GP145-TrkB/GP95-TrkB; trkB tyrosine kinase; Neural receptor protein-tyrosine kinase (trkB); Tkrb; trkB; TRKB1; RATTRKB1; |
Gene ID | 25054 |
mRNA Refseq | NM_001163168 |
Protein Refseq | NP_001156640 |
MIM | |
UniProt ID | |
Pathway | ARMS-mediated activation, organism-specific biosystem; Activation of TRKA receptors, organism-specific biosystem; Frs2-mediated activation, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved |
Function | ATP binding; brain-derived neurotrophic factor binding; brain-derived neurotrophic factor binding; brain-derived neurotrophic factor binding; brain-derived neurotrophic factor-activated receptor activity; brain-derived neurotrophic factor-activated recept |
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Not For Human Consumption!
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