Active Recombinant Rat NTRK1 protein, His-tagged

• Cat.No.: NTRK1-03R
• Product Overview: Recombinant Rat NTRK1 protein, fused to His-tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 35-418aa
• Description: TrkA, also known as Tyrosine kinase receptor A, is a member of the neurotrophic tyrosine kinase receptor(NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. It leads to cell differentiation and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as the establishment of thermal regulation via sweating. In one study conducted on two rat models, an inhibition of TrkA with AR786 led to a reduction in joint swelling, joint damage, and pain caused by inflammatory arthritis. Recombinant rat TrkA, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
• Form: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
• Bio-activity: Measured by ability to inhibit NGF-induced proliferation assay using TF‑1 human erythroleukemic cells in the presence of 0.5ng/ml of rat NGF. The ED50 range ≤ 5 ng/ml.
• Molecular Mass: 69 kDa
• AA Sequence: SCRETCCPVG PSGLRCTRAG TLNTLRGLRG AGNLTELYVE NQRDLQRLEF EDLQGLGELR SLTIVKSGLR FVAPDAFHFT PRLSHLNLSS NALESLSWKT VQGLSLQDLT LSGNPLHCSC ALLWLQRWEQ EDLCGVYTQK LQGSGSGDQF LPLGHNNSCG VPSVKIQMPN DSVEVGDDVF LQCQVEGQAL QQADWILTEL EGTATMKKSG DLPSLGLTLV NVTSDLNKKN VTCWAENDVG RAEVSVQVSV SFPASVHLGK AVEQHHWCIP FSVDGQPAPS LRWFFNGSVL NETSFIFTQF LESALTNETM RHGCLRLNQP THVNNGNYTL LAANPYGQAA ASIMAAFMDN PFEFNPEDPI PVSFSPVDTN STSRDPVEKK DETP
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
• Purity: > 90%
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: Ntrk1 neurotrophic receptor tyrosine kinase 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: NTRK1
• Synonyms: High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; NTRK-1; NTRK1; Slow nerve growth factor receptor; p140-TrkA; Trk-A; Trk; Trka; TRK1-transforming tyrosine kinase protein; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A
• Gene ID: 59109
• mRNA Refseq: NM_021589
• Protein Refseq: NP_067600
• UniProt ID: P35739