Active Recombinant Rat Jag1 Protein (Ser32-Asp1067), C-6×His-tagged

• Cat.No.: Jag1-12R
• Product Overview: Recombinant Rat Jag1 Protein (Ser32-Asp1067) with substitutions GGARN56-60AE and DR63-64TLVRPY and a C-terminal 6-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: Enables Notch binding activity and receptor ligand activity. Involved in several processes, including animal organ regeneration; inner ear auditory receptor cell differentiation; and positive regulation of cardiac epithelial to mesenchymal transition. Located in plasma membrane. Human ortholog(s) of this gene implicated in Alagille syndrome; congenital heart disease; multiple sclerosis; and tetralogy of Fallot. Orthologous to human JAG1 (jagged canonical Notch ligand 1).
• Source: Mouse myeloma cell line
• Species: Rat
• Tag: His
• Bio-activity: Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-2.4 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 114 kDa
  SDS-PAGE: 134-160 kDa, reducing conditions
• Protein length: Ser32-Asp1067
• N-terminal Sequence Analysis: Ser32
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: 12 months from date of receipt, ≤ -20 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: Jag1 jagged canonical Notch ligand 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Jag1
• Synonyms: Jag1; jagged canonical Notch ligand 1; protein jagged-1; jagged 1
• Gene ID: 29146
• mRNA Refseq: NM_019147
• Protein Refseq: NP_062020
• UniProt ID: G3V710