Active Recombinant Human JAG1, Fc tagged

• Cat.No.: JAG1-3138H
• Product Overview: Recombinant Human JAG1 (NP_000205.1) extracellular domain (Met 1-Ser 1046), fused with the Fc region of human IgG1 at the C-terminus, was produced in Human Cell.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met 1-Ser 1046
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells.
  The ED50 for this effect is typically 5-30 μg/mL.
• Molecular Mass: The recombinant human JAG1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 1254 amino acids and has a predicted molecular mass of 137 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human JAG1/Fc monomer is approximately 185.5 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Delivery of A Jagged1-PEG-MAL hydrogel with Pediatric Human Bone Cells Regenerates Critically-Sized Craniofacial Bone Defects (2023)

Gene Information

• Gene Name: JAG1 jagged 1 [ Homo sapiens ]
• Official Symbol: JAG1
• Synonyms: JAG1; jagged 1; AGS, Alagille syndrome , JAGL1; protein jagged-1; AHD; AWS; CD339; HJ1; AGS; JAGL1; MGC104644
• Gene ID: 182
• mRNA Refseq: NM_000214
• Protein Refseq: NP_000205
• UniProt ID: P78504

Reviews

01/01/0001

JAG1-3138H

Thanks for reaching out, I hope you’ve been well. Yes, your product worked as great as it always has for us, thank you for asking