Active Recombinant Rat Interleukin-17F

• Cat.No.: Il17f-498R
• Product Overview: Recombinant Rat IL-17F produced in E.coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 133 amino acids and having a molecular mass of 14.9 kDa.

Specification

• Cat. No.: IL17F-498R
• Description: IL-17F having an accession number of Q96PD4 is a cytokine that shares sequence similarity with IL17. IL-17F is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM-CSF. IL-17F inhibits the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. IL-17F induces stromal cells to produce proinflammatory and hematopoietic cytokines. Intestinal IL17F gene expression is increased in active CD.
• Species: Rat
• Source: E.coli
• Form: Lyophilized with no additives.
• Purity: Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: IL-17F is fully bioactive when compared to a known standard. The ED50 as determined by the dose-dependant induction of IL-6 in NHDF is 1.94 ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: MARRNPKVGL SALQKAGNCP PLEDNSVRVD IRIFNQNQGI SVPRDFQNRS SSPWD YNITR DPDRFPSEIA EAQCRHSGCI NAQGQEDGSM NSV PIQQEIL VLRREPQGCS NSFRLEKMLI KVGCTCVTPI VHH AA
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
• Tag: Non

Gene Information

• Gene Name: Il17f interleukin 17F [ Rattus norvegicus ]
• Official Symbol: Il17f
• Synonyms: Il17f; interleukin 17F; Il17f_predicted; LOC301291; MGC114402; interleukin 17F (predicted)
• Gene ID: 301291
• mRNA Refseq: NM_001015011
• Protein Refseq: NP_001015011
• UniPRot ID: Q5BJ95
• Chromosome Location: 9q13
• Function: cytokine activity; cytokine binding; protein homodimerization activity