Active Recombinant Mouse Il17a Protein

• Cat.No.: Il17a-095M
• Product Overview: Purified recombinant protein of Mouse IL-17A, the region of Ala26-Ala158, from gene Accession# NM_010552 and mouse IL-17F, the region of Arg29-Ala161, from gene Accession# NM_145856 without tag was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: 26-158;29-161 a.a.
• Description: This gene encodes a pro-inflammatory cytokine that is a member of the interleukin-17 family. The encoded protein plays a central role in host defense against diverse pathogens. The encoded protein is produced by activated T-cells and certain cell types of innate immune system. The active protein functions as either a homodimer with other interleukin-17 family members and signals through the interleukin-17 receptor to induce inflammatory cytokine production. Aberrant expression of this gene is associated with autoinflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
• Bio-activity: The ED50 is 125 - 175 ng/ml, corresponding to a specific activity 0.57 - 0.8 x 10^4 units/mg, determined by a dose dependent stimulation of fetal mouse skin fibroblasts production of IL-6.
• Molecular Mass: 30 kDa
• Endotoxin: Less than 0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Storage Buffer: 10 mM NaH2PO4, 0.15 M NaCl, pH7.2

Gene Information

• Gene Name: Il17a interleukin 17A [ Mus musculus (house mouse) ]
• Official Symbol: Il17a
• Synonyms: Il17a; interleukin 17A; Il17; Ctla8; IL-17; Ctla-8; IL-17A; interleukin-17A; cytotoxic T-lymphocyte-associated antigen 8
• Gene ID: 16171
• mRNA Refseq: NM_010552
• Protein Refseq: NP_034682
• UniProt ID: Q62386