Active Recombinant Rat Il18

• Cat.No.: Il18-436R
• Product Overview: Interleukin-18 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18260 Dalton. The IL-18 is purified by proprietary chromatographic techniques.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: IL-18 is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1mg/ml) solution in water containing 0.4 x PBS pH=7.2.
• Bio-activity: The ED50 as determined by the dose-dependant stimulation of IFN-gamma by murine lymph node cells (400,000cells/well) co-stimulated with sub optimal dose of ConA (0.75ug/ml) is 12ng/ml corresponding to a specific activity of 84,000U/mg. The medium was RPMI containing 10% FCS and 50µM 2-ME.
• AA Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be His-Phe-Gly-Arg-Leu.
• Purity: Greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
• Stability: Lyophilized Interleukin-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized Interleukin 18 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: Il18 interleukin 18 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Il18
• Synonyms: Il18; IL-18; interleukin 18; interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor
• Gene ID: 29197
• mRNA Refseq: NM_019165
• Protein Refseq: NP_062038
• UniProt ID: P97636
• Chromosome Location: 8
• Pathway: African trypanosomiasis; Cytokine Signaling in Immune system; Cytosolic DNA-sensing pathway
• Function: cytokine activity; interleukin-18 receptor binding; protein binding

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.