Active Recombinant Rat IL12A & IL12B Protein, His-tagged

• Cat.No.: IL12A & IL12B-1257R
• Product Overview: Recombinant rat IL23A (NP_569094.1) (Met1-Ala196) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant rat IL12B (EDM04146.1) (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the p

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 1-196;1-335 a.a.
• Predicted N Terminal: Val 22 & Met 23
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 2-15 ng/mL.
• Molecular Mass: The recombinant rat IL23A & the rat IL12B comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.2 (21+37.2) KDa. The apparent molecular mass of the rat IL23A & the rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: (17.9+53.5+2.9+23.8) % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Il12a interleukin 12A [ Rattus norvegicus ]
• Official Symbol: IL12A
• Synonyms: IL12A; interleukin 12A; interleukin-12 subunit alpha; IL-12A; CLMF p35; IL-12 subunit p35; interleukin 12 p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit
• Gene ID: 84405
• mRNA Refseq: NM_053390
• Protein Refseq: NP_445842
• Pathway: African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved
• Function: cytokine activity; contributes_to cytokine activity; growth factor activity; contributes_to growth factor activity; interleukin-12 beta subunit binding; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; interleu