Active Recombinant Rat Il10 Protein (160 aa)

Cat.No. : Il10-006I
Product Overview : Recombinant Rat Il10 Protein without tag was expressed in E. coli.
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Species : Rat
Source : E.coli
Protein Length : 160
Description : Interleukin-10 also known as cytokine synthesis inhibitory factor (CSIF), is coded by IL10 gene on chromosome 13 in rat. the charter member of the IL 10 family of α helical cytokines that also includes IL19, IL20, IL22, and IL24 (1, 2). IL10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNFα. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells.
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : Measured in a cell proliferation assay using MC/92 mouse mast cells. The ED50 for this effect is typically 2-12 ng/mL.
Molecular Mass : Approximately 18.6 kDa, a single non-glycosylated polypeptide chain containing 160 amino acid residues.
AA Sequence : SKGHSIKGDNNCTHFPVSQTHMLRELRAAFSQVKTFFQKKDQLDNIVLTDSLLQDFKGYLGCQALSEMIKFYLVEVMPQAENHGPEIKEHLNSLGEKLKTLWIQLRRCHRFLPCENKSKAVEQVKNDFNKLQDKGVYKAMNEFDIFINCIEAYVTLKMKN
Endotoxin : Less than 1 EU/μg of rRtIL-10 as determined by LAL method.
Purity : >97% by SDS-PAGE and HPLC analyses.
Storage : This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
Storage Buffer : Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name Il10 interleukin 10 [ Rattus norvegicus ]
Official Symbol Il10
Synonyms IL10; interleukin 10; interleukin-10; CSIF; IL-10; cytokine synthesis inhibitory factor; IL10X;
Gene ID 25325
mRNA Refseq NM_012854
Protein Refseq NP_036986
UniProt ID P29456

Not For Human Consumption!

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