Active Recombinant Rat GPC3 protein, His-tagged

• Cat.No.: GPC3-1305R
• Product Overview: Recombinant Rat GPC3 protein(Gln 25 - Met 557), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Rat Glypican 3, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag with a linear range of 0.6-20 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 62.4 kDa.
• Protein length: Gln25-Met557
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Gpc3 glypican 3 [ Rattus norvegicus ]
• Official Symbol: GPC3
• Synonyms: GPC3; glypican 3; glypican-3; proteoglycan GPC3; intestinal protein OCI-5; defective in Simpson-Golabi-Behmel overgrowth syndrome; OCI-5; MGC93606
• Gene ID: 25236
• mRNA Refseq: NM_012774
• Protein Refseq: NP_036906