Active Recombinant Rat Gfra1 protein(Met1-Leu445), His-tagged

• Cat.No.: Gfra1-4085R
• Product Overview: Recombinant Rat GFRA1 (Q62997-1) (Met 1-Leu 445) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Leu445
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.2-1 μg/mL in the presence of 40 ng/mL Recombinant Rat GDNF.
• Molecular Mass: The recombinant rat GFRA1 comprises 432 amino acids and predicts a molecular mass of 48 kDa. The apparent molecular mass of the rat GFRA1 is approximately 58-65 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Gfra1 GDNF family receptor alpha 1 [ Rattus norvegicus ]
• Official Symbol: Gfra1
• Synonyms: GFRA1; GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNFR-alpha; GFR-alpha-1; RET ligand 1; GDNFR-alpha-1; GDNF receptor alpha; GDNF receptor alpha-1; TGF-beta-related neurotrophic factor receptor 1; Glial cell line-derived neurotrophic factor receptor alpha; glial cell line derived neurotrophic factor family receptor alpha 1
• Gene ID: 25454
• mRNA Refseq: NM_012959
• Protein Refseq: NP_037091