Active Recombinant Rat Fibroblast Growth Factor 7

• Cat.No.: Fgf7-372R
• Product Overview: Recombinant Rat Fibroblast Growth Factor 7 was expressed inE.Coli.MW=17 kDa.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells.
• Purity: Purity > 90%. Purified by heparin affinity chromatography.
• Activity: The activity of rat FGF-7 at 1-10 ng/ml is determined by tritiated thymidine incorporation using cultured mouse keratinocytes. Maximum stimulation observed at 1 ng/ml. The optimal concentration of this product for use with other cell types must be determined by the end user.
• Specifity: 45102 binds to keratinocytes and most parenchymal epithelial cells; binds specific splice variant of FGF receptor expessed in epithelial cells (FGFr2IIIb).
• Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of IGF-II as a Reference Standard.
• Storage: This product is stable for at least one year at -20℃ to -70℃. Keep frozen until use. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: Fgf7 fibroblast growth factor 7 [ Rattus norvegicus ]
• Synonyms: Fgf7; fibroblast growth factor 7; Kgf; fibroblast growth factor 7; FGF-7; HBGF-7; keratinocyte growth factor; heparin-binding growth factor 7
• Gene ID: 29348
• mRNA Refseq: NM_022182
• Protein Refseq: NP_071518
• UniProt ID: Q02195
• Chromosome Location: 3q36
• Pathway: MAPK signaling pathway; Melanoma; Pathways in cancer; Regulation of actin cytoskeleton
• Function: chemoattractant activity; growth factor activity; heparin binding; protein binding; type 2 fibroblast growth factor receptor binding