Active Recombinant Rat Eng, Fc Chimera

• Cat.No.: Eng-638R
• Product Overview: Recombinant Rat Eng (Accession # NP_001010968) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Rat
• Tag: Fc
• Predicted N Terminal: Glu26
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA.When Recombinant Rat Endoglin/CD10^5 Fc Chimera is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.25-1.25 µg/mL.Measured by its ability to inhibit BMP-10-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 0.15-0.75 µg/mL.
• Molecular Mass: Recombinant Rat Eng, Fc Chimera has a calculated MW of 87.0 kDa (monomer). In SDS-PAGE migrates as 100-115 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Eng endoglin [ Rattus norvegicus ]
• Official Symbol: Eng
• Synonyms: ENG; endoglin; MGC124909
• Gene ID: 497010
• mRNA Refseq: NM_001010968
• Protein Refseq: NP_001010968