Active Recombinant Rat Defb4

• Cat.No.: Defb4-8655R
• Product Overview: Recombinant Rat Defb4 was expressed in E. coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four rat β-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney.
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH 7.4, 500mM NaCl.
• Bio-activity: Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml.
• Molecular Mass: Approximately 4.4 KDa, a single non-glycosylated polypeptide chain containing 41 amino acids.
• AA Sequence: QSINNPITCL TKGGVCWGPC TGGFRQIGTC GLPRVRCCKK K
• Endotoxin: Less than 1EU/μg of rRtBD-4 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at

Gene Information

• Gene Name: Defb4 defensin beta 4 [ Rattus norvegicus ]
• Official Symbol: Defb4
• Synonyms: DEFB4; defensin beta 4; beta-defensin 4; BD-2; BD-4; RBD-2; RBD-4; beta defensin-2; defensin beta 3; Defb2; Defb3
• Gene ID: 64389
• mRNA Refseq: NM_022544
• Protein Refseq: NP_071989