Active Recombinant Rat Cyp3a9 Protein

• Cat.No.: Cyp3a9-137R
• Product Overview: Recombinant rat Cyp3a9 protein and CYP-reductase without tag were coexpressed in E. coli.

Specification

• Species: Rat
• Source: E.coli
• Description: Catalyzes the hydroxylation of progesterone at 6beta, 16alpha, and 21 positions
• Bio-activity: Cytochrome c Reductase Activity: 806 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Testosterone 6β-hydroxylase
  Linear with time up to: 30 min(Determined at 32 µM substrate, using a P450 concentration of 50 pmol/ml)
  Linear with P450 up to: 100 pmol/ml(Determined at 32 µM substrate, using an incubation time of 5 min)
  Vmax: 3.4 pmol/min/pmol CYP
  Km: 21 µM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 5.5 nmol/ml
  Protein Concentration: 16.0 mg/ml
  Specific P450 Content: 344 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: Cyp3a9 cytochrome P450, family 3, subfamily a, polypeptide 9 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Cyp3a9
• Synonyms: Cyp3a9; cytochrome P450, family 3, subfamily a, polypeptide 9; Cyp3a13; P450olf3; cytochrome P450 3A9; 3AH15; CYPIIIA9; P450-OLF3; cytochrome P450 olfactive 3; cytochrome P450, family 3, subfamily a, polypeptide 13; cytochrome P450-OLF3; olfactive; olfactory cytochrome P450olf3
• Gene ID: 171352
• mRNA Refseq: NM_147206
• Protein Refseq: NP_671739
• UniProt ID: Q5PQX2