Active Recombinant Rat Cyp2d4 Protein

• Cat.No.: Cyp2d4-139R
• Product Overview: Recombinant rat CYP2D18 protein and CYP-reductase without tag were coexpressed in E. coli.
  Amount: 1 nmol cytochrome P450.

Specification

• Species: Rat
• Source: E.coli
• Description: Has N-demethylation activity toward the antidepressant imipramine
• Bio-activity: Cytochrome c Reductase Activity: 1531 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Dextromethorphan O-demethylase
  Linear with time up to: 6 min(Determined at 15 µM substrate, using a P450 concentration of 20 pmol/ml)
  Linear with P450 up to: 40 pmol/ml(Determined at 15 µM substrate, using an incubation time of 5 min)
  Vmax: 5.1 pmol/min/pmol CYP
  Km: 39 µM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 2.4 nmol/ml
  Protein Concentration: 11.3 mg/ml
  Specific P450 Content: 212 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: Cyp2d4 cytochrome P450, family 2, subfamily d, polypeptide 4 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Cyp2d4
• Synonyms: Cyp2d4; cytochrome P450, family 2, subfamily d, polypeptide 4; Cyp2d6; Cyp2d18; Cyp2d22; Cyp2d4v1; Cyp2d4v2; cytochrome P450 2D4; CYPIID18; CYPIID4; Cytochrome P450, subfamily IID4; P450 2D-29/2D-35; P450-CMF3; P450-DB4; cytochrome P450 2D-29; cytochrome P450 2D-35; cytochrome P450 2d18; cytochrome P450, family 2, subfamily d, polypeptide 22; cytochrome P450, subfamily 2D, polypeptide 6; cytochrome P450, subfamily IID, polypeptide 6; cytochrome P450-CMF3; cytochrome P450-DB4; debrisoquine 4-hydroxylase
• Gene ID: 171522
• mRNA Refseq: NM_138515
• Protein Refseq: NP_612524
• UniProt ID: Q64680