Active Recombinant Rat Cd47, His tagged

• Cat.No.: Cd47-8767R
• Product Overview: Recombinant Rat Cd47 (P97829-1) (Met1-Lys140), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Lys140
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by its binding ability in a functional ELISA.
  Immobilized Rat CD47-His at 10 μg/ml (100 μl/well) can bind mouse SIRPA-Fc, The EC50 of mouse SIRPA-Fc is 0.6-1.4 μg/ml.
• Molecular Mass: The recombinant rat CD47 comprises 133 amino acids and predicts a molecular mass of 15.2 kDa. The apparent molecular mass of the recombinant protein is approximately 27-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd47 integrin-associated protein [ Rattus norvegicus ]
• Official Symbol: Cd47
• Synonyms: CD47; integrin-associated protein
• Gene ID: 113934